Histamine Stimulated Small Intestine

Histamine Stimulated Small Intestine The longitudinal smooth muscle of the guinea-pig ileum small intestine contracts in response to acetylcholine. These contractions can be reduced by the application of adenosine and related compounds. The guinea-pig ileum is innervated by the enteric, sympathetic and parasympathetic divisions of the autonomic nervous system. The parasympathetic and enteric fibres release acetylcholine which acts on muscarinic receptors. The action of adenosine and its receptor antagonists can be assessed by comparing electrically induced contractions via electrical field stimulation and histamine induced contractions. Electrical field stimulation contractions cause presynaptic release of acetylcholine to produce contractions where as the histamine induced contractions cause postsynaptic contractile responses. Throughout this study adenosine and its receptor antagonist actions will be investigated and compared using electrical field stimulation and histamine. The contraction of the gut In gastrointestinal smooth muscles, researches show that there are two types of muscarinic receptors types that are present as targets to the neurotransmitter acetylcholine (Okamoto et al., 2002).Acetylcholine and its derivatives produce contractions by activating muscarinic receptors. The muscarinic receptors types are known as M2 and M3. Binding Studies have portrayed that the number of M2 receptors is greater than that of the M3 receptors however functional studies have shown that M3 muscarinic receptors play a fundamental role in mediating the contractile response (Eglen et al., 1996) and the functional role of M2 exists as unclear (Clague et al., 1985). The M3 receptor is coupled with G proteins, causing activation of phospholipase C and formation of inositol trisphosphate and diacylglycerol, which are expected to contribute in muscarinic receptor mediated smooth muscle contractions (Unno et al., 2005). They also mediate relaxation due to the release of nitric oxide from neighbo uring endothelial cells. M3 receptors in visceral smooth muscle contribute to the smooth muscle stimulating effect of muscarinic agonists. However the muscarinic receptor most abundant in the ileum is the M2 which cause an indirect contraction of the guinea-pig ileum by preventing the relaxing effect of drugs (Ehlert and Thomas, 1995). Both muscarinic receptor subtypes are activated by acetylcholine and produce a contractile response; however they vary in their transduction mechanisms and signalling pathways. Adenosine Adenosine has numerous diverse roles in normal physiology; such roles include promoting/maintaining sleep, regulating state of encouragement as well as local neuronal excitability and coupling cerebral blood flow to energy demand (Dunwiddie and Masino, 2001). It exists free in the cytosol of all cells and is transported in and out of all cells mainly using a membrane transporter(Rang et al., 2007). Under normal conditions, adenosine is formed intracellularly as well as extracellularly (Fredholm et al., 2001). ATP is stored in vesicles and released by exocytosis. It is also available in the cytosol of cells and is taken up and released via a specific membrane transporter. Released ATP and ADP are rapidly converted to adenosine by the action of tissue nucleotides. Studies have shown that there are pathways that contribute to adenosine formation, a) by the action of adenylate kinase and cystolic 5-nucleotidase, b) formation from hydrolysis of adenosine 3, 5 phosphate and c) formation by the action of S-adenosylhomocysteine (SAH) hydrolase. The pharmacological effects of adenosine include smooth muscle relaxation and inhibition of nerve activity, lipolysis and platelet aggregation(Daly et al., 1983). There is evidence that stimulation or inhibition is of adenylate cyclise is involved in adenosine action and therefore it has been concluded that adenosine is mediated by cyclic AMP. Based on its ability to inhibit cell function and thus minimise the metabolic requirements of cells, one of its functions may be as a protective agent released when tissue integrity is threatened. Adenosine exerts its physiological actions activation of a number of specific cell surface receptors. There are four different adenosine receptors known as A1, A2A, A2B, and A3. Some characteristics of these receptors are presented in Figure 1a. These subtypes have been distinguished on the basis of their agonist and antagonist selectivity. They belong to the G protein-coupled receptors. Mechanism of Adenosine action Adenosine A1 receptors are negatively coupled to the inhibition of adenylate cyclase, however they can act through other pathways such as stimulation of phospholipase C, activation of potassium channels and inhibition of N-type calcium channels (Zizzo et al., 2009). A1 receptors are coupled to Gi and G0 proteins and lead to inhibition of adenylate cyclase and consequently cause a decrease in cAMP (Ranjit, 2008). Adenosine A2A and A2B are coupled for activation of adenylate cyclise whereas A3 receptors have been shown to stimulate phospholipase C and D, to inhibit adenylate cyclase and to activate ATP sensitive potassium channel (Ralevic and Burnstock, 1998). Activation of these receptors require comparatively high amounts of adenosine. A2A and A2B receptors have a high and a low affinity for adenosine respectively. Receptor Subtype A1 A2A A2B A3 Transduction mechanism Inhibits adenylyl cyclase Activates adenylyl cyclase Activates adenylyl cyclase Inhibits adenylyl cyclase Primary distribution Brain (cortex, cerebellum, hippocampus). Dorsal horn of spinal cord. Eye, adrenal gland, atria Spleen, thymus, leukocytes, blood platelets. Straitopallidal GABAergic neurons, olfactory bulb Caecum, colon, bladder Testis, mast cells Tissue functions Antinociception, Hypothermia. Sedation, Sleep, Inhibition of lipolysis, Cardio and neuroprotection Reflex tachycardia, vasodilation, inhibition of platelet aggregation, sleep protection against ischemia Relaxation of vascular and intestinal smooth muscle, cytokine production, inhibition of cell proliferation Mast cell degranulation, coronary vasodilation and protection from reperfusion Selective antagonists DPCPX PSB 36 SCH 58261 PSB 1115 potassium salt MRS 3777 hemioxalate Figure 1a: Summary of adenosine receptors. Adenosine and the enteric functions of the Gut The enteric nervous system (ENS) consists of a compilation of neurons in the gastrointestinal nervous system which is capable of functioning independently of the central nervous system. It moderates motility, secretion, microcirculation, inflammatory and immune responses of the gastrointestinal tract (Altaf and Sood, 2008). The ENS is composed of extrinsic, which consists of parasympathetic and sympathetic divisions and the intrinsic component which encloses neurons. Intestinal functions results from an interaction between the ENS, smooth muscle and the mucosal/immune system. The network is regulated by several mediators; however there is consolidating evidence that adenosine is a significant regulating agent (Bueno, 2000) (Wood, 2004). Studies show that in the small intestine adenosine and adenosine derivatives where found to inhibit cholinergic transmission in guinea-pigs via a prejunctional action on neurotransmitter release (Gustafsson et al., 1978). The action of the A1 receptor s allowed mediation of the inhibitory action of adenosine in the cholinergic transmission(Shinozuka et al., 1985) of motor neurones innervating circular and longitudinal smooth muscle however A2A receptors have been reported to reduce the cholinergic motor responses(Gustafsson et al., 1985a; Gustafsson et al., 1985b). Histamine Histamine has a role as a primary transmitter or neuromodulator and it is widely distributed within mammalian tissues. (Izzo et al., 1998). Histamine is a vasoactive substance to be identified in the body which can rapidly metabolise and holds properties of being highly polar and not diffusing readily across cell membranes or the blood-brain barrier. It is stored in mast cells and basophils of blood and has two receptors known as H1 and H2. The release of histamine could cause changes in the cardiovascular system and induce anaphylactic shock. Histamine has been shown to induce gastric acid secretion through the H2 receptors linked to cyclic AMP production in oxyntic cells. Researches show that gastric cells of the guinea-pig may have a class of binding sites for histamine which shows no relationship to adenylate cyclase and the H2 receptor. Histamine creates a spasmogenic effect on the intestine that results from H1 receptor stimulation(Guy A. and Settipane, 1988-1989). There are three histamine subtypes known as H1, H2 AND H3 and all three have been identified to be present in the guinea pig small intestine. Studies show that H1 receptor subtypes mediate the contraction of the longitudinal muscle in the small intestine (Izzo et al., 1998). However researchers also state that the effect of histamine is predominantly due to the interaction with H1 receptors located on smooth muscle cells and moderately due to the interaction H2 receptors present on myenteric plexus interneurones (Bauer and Matusak, 1988). AIMS The aim is to confirm the prejunctional action of adenosine and examine whether adenosine has the additional ability to relax the smooth muscle directly. The project will use histamine to contract the smooth muscle and the objective is to find out whether adenosine can reduce these contractions and if so is the concentration range similar to that needed to inhibit the contractions to the electrical field-stimulation? It will also be investigated what adenosine receptor subtype is involved (A1, A2A, A2B, A3 identified using selective antagonists). METHOD Animals and preparation of tissue Dunkin Hartley guinea-pigs (250g +) of male sex that had previously been fed Harlan 2040, the guinea-pig diet and ad lib filtered tap water, were obtained from Harlan UK. They were group housed and provided with grade 6 woodchip and hay bedding. Their enrichment consisted of plastic and cardboard fun tunnels, plastic igloos and gnawing blocks. Furthermore they were kept at room temperatures of 19-23 °C and at room humidity of 45-65%. They were provided with 12 hours light and 12 hours of dark lighting. The guinea-pigs were stunned by a blow to the head and sacrificed by exsanguination. Two segments of 3cm length were removed from the distal part of the small intestine, for each tissue the ends were tied with cotton threads to the tissue holder and then suspended in 20ml organ baths containing Krebs solution (composition in mM: NaCl, 118; NaHCO3, 25; Glucose, 11; KCl, 4.7; CACl2, 2.5; KH2PO4, 1.18; MgSO4, 1.18). This was aerated with 95% O2 and 5% CO2 and maintained at 37 °C. The tissues were left for 30minutes to equilibrate under a resting tension of 1g before starting the stimulation. Experimental protocol The organ baths were equipped with parallel electrodes which allowed electrical field stimulation to be transmitted at a frequency of 0.1Hz, 40V, 0.5ms pulse duration. Contractions of the ileum were measured with isometric transducers (ADInstruments Force Transducers), amplified and recorded onto a data capture system (Lab Charts on the PC). The tissues were allowed to stabilise in the organ baths in order to reach steady contractions. Figure 1b represents the experimental set up. C:UsersHomeDocumentsDSC00308.JPG Figure 1b shows the laboratory designed set up for the experiment, with a set of two organ baths. There were several parts (a-e) to the experiments to be carried out on the ileum. n is the number of ileum used throughout the experiment n=18. The number of experiments carried out on the ileum was 56. Effect of Adenosine-the experiment consisted of stimulating the tissues continuously with electrical field stimulation and adding cumulative concentrations of adenosine (10-11M 10-4M) to the organ baths and the responses were recorded. Effect of Histamine- the tissues were stimulated with histamine, cumulative concentrations of histamine (10-11M 10-4M) were added to the organ bath and responses were measured. The concentration 110-6M gave the maximum response and a steady contraction, it was used to contract the tissue with adenosine. Effect of Adenosine in the presence of Histamine- the concentration of histamine that gave the maximum and steady response was added to organ bath. The tissue was permitted to stabilise in order to reach steady contractions. Once reached, cumulative concentrations of adenosine were added (10-8M 10-4M) in order to observe the responses of the ileum to adenosine in the presence of histamine. Effect of PSB36(10-8M) SCH58261(10-7M) to confirm identity of receptors being investigated, cumulative dose-response curves for induced contractions by histamine were observed for adenosine in the presence of selective antagonists, PSB36 and SCH58261. An experiment was also carried out, which involved electrical field stimulation to contract the tissue, adenosine was added in the presence of these antagonists. Effect of Atropine Atropine concentrations of 10-6M 10-7M were added to establish the effects of atropine on the electrically field stimulated ileum. For each drug that was being tested except atropine, the experiment was repeated at least six times. The tissue was washed out three times at least after an experiment was completed and was allowed to recover before another experiment was carried out. The electrical field stimulation was also switched off each time the tissue was washed out. Chemicals and drugs The drugs that were used consisted of Adenosine hemisulphate salt, Histamine diphosphate salt and Atropine sulphate salt which were all purchased from Sigma-Aldrich, Poole, UK. PSB36 and SCH58261 and DMSO (Dimethyl sulfoxide) were purchased from Tocris -Cookson, Bristol, UK. All drugs were dissolved in distilled water with exceptions of PSB36 AND SCH58261 which were diluted with DMSO.10ml of stock solution were made up in each case. Statistical Analysis All drug concentrations presented were final bath concentrations. The drug effects were expressed as twitch contraction (g). All data were given as means  ± S.E.M, where n represents the number of animals from which tissues were taken and on which observations were made. Inhibitory effects by adenosine in the field stimulated guinea pig ileum were measured and the responses of ileum twitch contraction were recorded for each concentration applied. This was repeated when using adenosine receptor antagonists. Adenosine responses were fitted onto concentration-response curve. Effects of histamine stimulated guinea pig ileum were also recorded. Adenosine responses and its receptor antagonist response were measured and plotted. 3.0 RESULTS a. b. Figure 2. The effect of adenosine concentrations in the guinea-pig ileum. Data are means  ±S.E.M and are expressed as an average of contractions (g). a. Representative traces showing inhibitory responses induced by adenosine. b. Concentration response curve for adenosine representing the average twitch response (g) when cumulative adenosine concentrations were added. Each point with bar represents the mean  ± S.E.M (n=6). Figure 3a: Original trace illustrating twitch response abolished by atropine 10-6M final bath concentration. b. c. Figure 3. Influences of atropine and potent and selective A1 adenosine receptor antagonist PSB36 10-7M AND 10-8M on guinea-pig ileum. b. Twitch responses of the guinea-pig ileum preparation to electrical field stimulation in the presence of PSB36 10-7M (n=6) and PSB36 10-8M (n=4), compared to twitch responses to adenosine alone. The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. c. Contractions of the guinea-pig ileum preparation stimulated by histamine in the presence of PSB36 10-7M (n=6) and PSB36 10-8M (n=4), compared to responses to adenosine when stimulated with histamine (n=6). The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. a. b. Figure 4. Summarises the effect of potent and selective A2A adenosine receptor antagonist SCH58261 10-7M on guinea-pig ileum. a. Twitch responses of the guinea-pig ileum preparation to electrical field stimulation in the presence of SCH58261 10-7M (n=6), compared to twitch responses to adenosine alone. The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. b. Contractions of the guinea-pig ileum preparation stimulated by histamine in the presence of SCH58261 10-7M (n=6), compared to responses to adenosine when stimulated with histamine (n=6). The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. Figure5a. A concentration-response curve illustrating the effects of adenosine on the guinea-pig ileum when the tissue is stimulated with histamine. . Each point with bar represents the mean  ± S.E.M (n=6). Figure 5b. Responses of the guinea-pig ileum to cumulative concentrations of histamine. Each point with bar represents the mean  ± S.E.M (n=8). Electrical field stimulated guinea-pig ileum produced reproducible twitch responses to adenosine. Adenosine concentrations ranging from 10-8M final bath concentration caused concentration dependent inhibitory effects. Administration of increasing adenosine concentrations decreased electrically evoked acetylcholine release from the cholinergic nerve endings of the ileum (Figure2). Figure 2b clearly portrays that adenosine has no effect at low final bath concentrations of 10-11M. Atropine 10-6M final bath concentration abolished the twitch responses to electrical field stimulation confirming they were produced by cholinergic nerve stimulation (Figure3). In the presence of selective A1 adenosine receptor antagonist PSB36 10-7M and 10-8M concentration and electrical field stimulation, the twitch responses to adenosine decreased, when adenosine was applied at higher concentrations, the twitch responses became smaller as acetylcholine release was inhibited and completely prevented by PSB36. Figure 3a shows the curve shifted to the right when PSB36 10-8 was administered compared to the curve of adenosine. When PSB36 10-7 was applied there is a slight shift of the curve to the right compared to the adenosine curve. The effects of the antagonist PSB36 are seen significantly at 10-8M final bath concentration as the shift of the curve is greater. The highest response the ileum produced was as at adenosine concentration of 1 x 10-8M, the responses lowered slightly at 1 x 10-6M and consequently decreased rapidly at 1 x 10-5M final bath concentrations. Histamine induces contractions in the ileum. Addition of cumulative concentrations of adenosine in the presence of PSB36 10-7M produced small responses compared to the contractions produced in the presence of PSB36 10-8M. The effect of PSB36 10-8M caused a rightward shift compared to the curve representing the effect of adenosine in the presence of histamine (Figure 3b). Electrically field stimulated ileum in the presence of the selective adenosine receptor competitive antagonist SCH58261 produced smaller responses (Figure 4a). Following administration of cumulative concentrations the responses decreased however produced no right shift in the dose response curve. Histamine excites the tissue causing it to contract and producing a high response, when adenosine is applied in the presence of SCH58261, the responses are inhibited and acetylcholine release is decreased and thus there is a decrease in the shape of the curve in Figure 4b. The results expressed in Figure 5a illustrate the inhibitory effects of adenosine in the ileum. Figure 5b illustrates the effects of histamine concentrations on the ileum. The experiment was carried out to investigate the best concentration to use so that a maximum and strong, reproducible contraction would be produced; the figure confirmed 110-6M final bath concentration to give the highest and steady contraction of the tissue. Moreover this permitted to obtain a dose-response curve for adenosine with histamine providing a suitable starting concentration for each drug at 110-8M. DISCUSSION The results of this present study show that adenosine plays an inhibitory role on muscular contractility in guinea-pig ileum. Adenosine prevents the neuroeffector transmission in guinea-pig ileum. The action of adenosine appeared to be cholinergic prejunctional in nature, this is portrayed when adenosine is applied to electrically field stimulated guinea-pig ileum(Gustafsson et al., 1985b). The action of adenosine on histamine stimulated guinea-pig ileum in the absence and presence of adenosine antagonists also indicates reduction in neuroeffector transmission however due to postjunctional action. The effect of adenosine on the guinea-pig ileum can be observed in Figure 2. Adenosine produced a dose-dependent depression on the response. It reduced the electrically evoked acetylcholine release from the ileum. A1 and A2 receptors have been reported to reduce acetylcholine release in the gastrointestinal tract(Tomaru et al., 1995). Adenosine released from neuronal endings is thought to have direct actions on smooth muscle as they illustrate relaxant neurotransmitters in the gastrointestinal tract(Storr et al., 2002). Atropine is a competitive antagonist for the muscarinic acetylcholine receptor, consequently when applied to the ileum at 10-6M final bath concentration; there is rapid inhibition of response confirming that the twitch responses were produced by cholinergic nerve stimulation. Adenosine inhibited the twitch response of the electrically stimulated guinea-pig ileum preparation, in the presence of PSB36 10-8M final bath concentration there was a right shift in the adenosine curve thus interpreting that higher concentrations were required to lower the twitch response. The general trends that Figure 3a displays is that the curves have the same form; the linear proportions of the curves are parallel. The traces help to show the changes in the response curve to adenosine and adenosine selective receptor antagonist PSB36. There is a slight fall in tension when adenosine 310-7M was applied in the presence of PSB36 10-8M; however there was a rapid decrease when adenosine 110-5M was administered causing the right shift in the Figure. Upon cumulative additions of adenosine to field stimulated guinea pig ileum the concentration required to inhibit acetylcholine release was of 310-7M, the effects diminish once concentration of adenosine 110-5M was added providing evidence that adenosine has the ability to relax smooth muscle in the ileum. Since PSB36 is a potent and selective A1 adenosine receptor antagonist, the A2A receptors increase electrically induced twitch contractions in the guinea pig ileum, which contributes to assistance of acetylcholine release (Storr et al., 2002). The effects of adenosine in the presence of PSB36 10-7M 10-8M to histamine stimulated guinea pig ileum can be observed in Figure 3b. Histamine administration to the guinea pig ileum caused a tonic histamine contraction which was followed by after-relaxation response, and application of adenosine inhibited the acetylcholine release. The ileum responses were reduced significantly with lower concentrations of adenosine in the presence of PSB36 10-7M and 10-8M compared to the concentrations of adenosine required in the electrically field stimulated ileum confirming that A1 receptors are the subtype present in the guinea pig ileum which cause the inhibition of acetylcholine release. SCH58261 did not significantly affect the position of the rightward shift however additions of cumulative adenosine concentrations caused reduction in the tension produced by the ileum. Large standard error bars can be observed in the figures, these may be due to human handling errors, i.e. micropipetting errors, administrating less or more concentration of adenosine or antagonists. Protein build up causing contamination in organ baths can also contribute to acquiring inaccurate results. Particular cells of the tissue may have become inactive at that moment of time. Furthermore it could be that the piece of thread holding the tissue may have become loose i.e. equipment errors and consequently tension was not measured accurately. It could also be due to unknown errors. Evidence that adenosine inhibits cholinergic neuroeffector transmission in the ileum by a prejunctional action on acetylcholine release can be of functional importance as adenine compounds are released during stimulation of intestinal nerves (Tomaru et al., 1995). Antagonists selective for adenosine receptors are beneficial in the research treatment of numerous conditions including cardiovascular, neurodegenerative and inflammatory diseases. In summary, the present study has confirmed the existence of presynaptic A1 receptors on the parasympathetic nerve terminals in the guinea pig ileum which upon activation causes inhibition of electrically induced neurogenic, cholinergic twitch contractions.

Causes of high unemployment in Zimbabwe Essay

Introduction: The unemployment rate is the proportion of the economically active population that is unemployed and actively looking for employment. In 1990, Zimbabwe embarked on a programme of Economic Reforms popularly known as Economic Structural Adjustment Programme (ESAP) and 1991-1992 saw one of the worst droughts. With close to 70% of the population living in the rural areas (CSO 1992) and dependent on agriculture for their livelihood, this constituted a major disaster. One result of these events was increased migration to the urban areas by people in search of employment. A further drought in 1995-1996 compounded the effect. Worsening economic conditions plus ever increasing corruption and crime have been the norm since 1995. Unemployment is currently soaring and the current rate stands at 70%. This has been from retrenchments, business closures e.t.c. According to EMCOZ survey, about 6000 jobs in the different sectors of the economy were lost by the end of 2001. The figure for 2002 was much higher as a result of the worsening business environment as the government fails to create opportunities to stimulate industrial expansion. Causes of Unemployment Unemployment is Zimbabwe has been caused by quite a number of factors. Most of these are mainly because of political reasons. The following are some of the factors that are causing unemployment: Sluggish investment and growth Weak export performance Poor macroeconomic policy environment The investment/business climate is unfriendly Population growth rate/age structure The growth path – reliance on primary products – is suboptimal Geography (proximity to South Africa, no direct access to the sea) Tertiary Education These are now explained below: Sluggish investment and growth The investment and growth of the Zimbabwe Economy has been shrinking of late. This has had a significant impact on the Zimbabwean companies. In the past 3 years, over 500 companies closed operations because of prohibitive operational conditions characterized by high input costs, hard currency shortages, high levels of interest rates, inflationary levels as well as the uncompetitive export market. Both the company and the government has been failing to effectively invest and thereby slowing growth. As a result, this has resulted in massive unemployment. Weak export performance Usually governments and exporters worry most about the foreign elasticity of demand for the exports. But in Zimbabwe today the most important is undoubtedly the domestic elasticity of supply of exports. This is reflected in the export volume numbers. In 2003, tobacco prices are still virtually unchanged from last year and higher than in previous years, but volumes are down by two-thirds. This then means that if we have very few goods that we are exporting, no we end up having a weak generation of foreign currency which we direly need for all our imports. This has significantly impacted on most organizations who eventually resort to the black market for the foreign currency. Poor macroeconomic policy environment The role of monetary policy is crucial because macroeconomic policy affects  the REAL economy, largely through the credit and capital markets. At the start of the 21st century monetary policy is the most powerful weapon that governments have at their disposal. Monetary policy influences interest rates (directly) and exchange rates (indirectly). It also influences investment, and thereby economic growth and employment. It has major implications for the financial markets – bonds and equities And today, its most important role is that of ensuring price stability. All this seem to have been ignored in the Zimbabwean scenario, where the macroeconomic policies are arm twisted just for the sake of political expediency. As a result, many sectors have been affected, and no jobs were created and thus an increase in unemployment. The investment/business climate is unfriendly The more profitable and attractive the business investment climate, the more firms will want to raise new money to expand and the more they will issue bonds. During a business boom or upswing the supply of bonds rises. The investment climate in Zimbabwe is not sufficiently favorable to attract the type of Foreign Direct Investment needed to transform exports. There are so many regulations concerning capital repatriation and profit/dividend remittability. This discourages investment and hence at the same time increasing unemployment. Population growth rate and age structure The population in Zimbabwe has been increasing at a decreasing rate, but the fact that the population is increasing without a complimentary increase in job opportunities, this therefore means all the job candidates are jobless. The growth path – reliance on primary products – is suboptimal Zimbabwe has been relying mostly on the products which are just primary. Such dependence has had no joy being found for the thousands of youths in Zimbabwe who are looking for jobs. Geography (proximity to South Africa, no direct access to the sea) Zimbabwe is a landlocked country and has been relying mostly on its neighbours namely South Africa and Mozambique. This has placed the country in a state of disadvantage hence the employment creation rate going down. Tertiary Education A number of tertiary colleges have sprung up in Zimbabwe, and yet the existing infrastructure is unable to absorb the swelling numbers of youths seeking training opportunities. There is also a high mismatch of skills acquired from training on one hand and the labour market opportunities on the other has led to increased job seekers. Seriousness of the problem: The issue of unemployment in Zimbabwe has reached such alarming levels which have brought discontent among the citizens of the country. This has been evidenced by the massive increases in the crime rates and other issues which include prostitution. This whole lot is as a result of a number of job seekers who are on the market failing to secure anything. Effects of Land Resettlement and HIV-AIDS on unemployment HIV and AIDS has been much talked about and discussed in Zimbabwe. This deadly incurable disease and virus has brought about much untold suffering and orphans. This also means that the number of job seekers flowing into the market will fall sharply. This might be a blessing in disguise in that it will provide a partial solution, but it will also highly likely slow the growth rate of the economy and thereby the rate of job generation. The issue about the effect of HIV-AIDS is two fold as it erodes both the employed and the unemployed. In the long run, there might end up there being very few numbers seeking for employment. The high levels of people who are  joining the job market make this a good pessimism. The massive land reform initiated by so-called war vets and the government produces more losers than winners. More than half a million farm workers have already lost their jobs and source of income, without gaining any of the redistributed land. Those displaced from the farms have found themselves on the job market. This then means that instead of solving a problem, the government has created more problems for itself. The problem with that is that this produces a chain of other problems interrelated to the farming business. On another note, if the land resettlement programme was well managed, this could bring a partial solution to the problem. This could temporarily create self-sustaining jobs for the rural community. The only problem is that the government in principle banned all commercial agriculture and villagised the whole country into small villages. This can never be a lasting solution to the unemployment problems as agriculture’s share of total employment falls as economies develop. Thus in the long run, this ceases to be a solution. Many hopes are on the informal sector which people say could expand given enough resources and incentives. Unfortunately, unemployment has risen far beyond the capacity of formal sector establishment while employment in the informal sector has grown phenomenally. Others, however, argue that expansion of the informal sector would generate new and equally serious economic problems – destroying the viability of formal sector operations, worsening crime levels, and devastating the environment, negatively affect national health programmes and affect state revenue inflows. Those in the informal sector remain an economic burden for the country because their incomes cannot meet basic needs and that most, if not all, operators subsist below the poverty datum line. It is a pre-requisite for employment creation in Zimbabwe to be enhanced by mobilisation of resources in capital markets through an unprecedented improvement of savings as a percentage of the Gross Domestic Product (GDP)  to around 25 percent so that funds would be invested and hence forth job creation. But to achieve this, the country should offer competitive investment incentives such as consistently low inflation and interest rates, stable foreign exchange rates, minimum state controls, low taxation and a politically stable environment. Improvement of conditions of services in the sector could be sustainable because of low wages, and there is need to realise that the economy could be stimulated through the rural sector, small scale and medium enterprises or large corporations. Conclusion All the stake holders, citizens and the government must all be actively involved the reduction of unemployment. Without this combined effort and commitment, unemployment is likely to continue rising since the economic conditions are worsening and it is becoming more difficult for the companies that had survived before to continue surviving. If things are left unattended to as they at the moment, unemployment will grow to be a self created time-bomb for the government and will eventually build enough pressure in it to force it to explode.

Meaning of life †Human Essay

?According to Albert Camus â€Å"You will never be happy if you continue to search for what happiness consists of. You will never live if you are looking for the meaning of life. † People can define happiness in various ways, for ones it means achieving their set goals while for others happiness means serving for society, and finally some people interpret happiness as finding the meaning of their life. People can spend their whole time only searching for the meaning of life, and they forget or ignore the life and real time that passes by. Unfortunately, you can search for the meaning of life and still not find it, or it can come to you with age. Significant events such as surviving the airplane or car crashes or losing the important people in one’s life, force humans to reconsider their values or beliefs, and can help to find the true meaning of life. Meaning of life is something that comes with the age, wisdom or experience. It is also hard to define what the meaning of life is, for it can be easily misinterpreted with the goals or values. I do not have my own meaning of life yet, for I am young, I have not so much experience in my life, and I cannot actually define what the meaning of life is. To begin with, I am not fully mature as a person because I am only twenty years old and my life has just started. Consequently, wanting to enjoy all the charms of my young life I try not to think about difficult topics such as meaning of life. I value my time that I have now, so I want to spend it learning and gaining an experience. The only thing I can figure out in this research on meaning of life, is that people value one thing – time. Having time we can find something or someone, develop ourselves and etc. Theoretically, we can do everything when we have time, but when we have time and do not have someone for whom we can spend it – our life becomes meaningless. Life becomes meaningful when we can spend time on objects and subjects that are important for us. As I am living with my parents, I do not even think about how to earn money, rise future generation and become independent person. I do not have to be a breadwinner at home, and I do not have to work yet. I do not have children, so I do not take the responsibility for treating my children right. However, it would be helpful to find out the meaning of life before I become a parent, for it would help me to raise my children right. My life is easier now, so I do not stop to think about the meaning of my life. Although I am dependent on my parents because they give me shelter, food and opportunity to get an education, I feel that being independent also would lead to thoughts about the meaning of life. Being a young adult give me the opportunity to gain an experience that could help me find the meaning of life. I am learning at the LCC university where professors from different countries tell their stories about their own life experience, and it seems that the person should have an experience in order to have a meaning of life. The experience of meeting different people, being introduced to different cultures, beliefs and values, and seeing the real adult life, all these experiences bring an insight about the meaning that the person can find in life. It is very difficult to find the right definition of meaning of life. When persons speak about creation of family or being a successful in work, it seems like goals and it is, but some people see it as the meaning. I can also assume that being happy and appreciative person has the real meaning in life, but is it the meaning of life? Maybe we should find the meaning that would lead us to feel happy and appreciative. I think that meaning of life does not even exist. There are only purposes. Love, family, friendship – are only purposes, and purpose is not the meaning. Why? Because they answer the question of approachability, we can reach it or not. Theoretically, if these things were meaningful, life would end when we reach all of them. Meaning is sort of general idea, which is accessible and equal to all. It cannot answer the question of feasibility. Actually, the meaning of life should by absolute verity. Overall, this is the topic that brings many thoughts to my mind. For now I can say that I do not have any specific meaning of my life so far. I am young, rebel, adventure seeking person, and the spirit that now lives in my soul gives me the opportunity to live and enjoy the fruits of my young adulthood years, and I rarely stop to think about the real meaning of life. I do not know whether it is right or wrong that I do not pay much attention for such topic. I believe that people become wiser with years, and that wisdom would bring the need to evaluate the life and to seek and find the meaning of it. I also cannot actually define what the meaning of life is, and I think that some people cannot find out it through their life. Overall, life is already meaningful as the person lives and breathes.

Civil Rights Movements During The World War II - 911 Words

Civil Rights Movements After the World War II, the United States has a significant impact in social changes on minorities’ social class status and gender. Women were not given equal rights to men and segregation in school between African American and White people to raise the issues to the roof. Veterans returned from the War had been treated badly and considered a lower class in society. Civil Rights movement involved many minorities group of people in the United States fight for the equal rights had been guaranteed in the Constitution, against racism, social class, and gender. It ended the segregation, racism, and gave society a different perspective on minority group of people in the United States. During the World War II, women played a role in the industrial and military through the workforce, helping the United States in building the country while in War and support troops from the warfare fighting for the nation. After the World War II, women had to lose their jobs over the veterans back from the War. Women were seen as no skill workers, home moms, and housewives. Of all the beneficial of post war, none of them rewards to women since veterans took more than the fifty percent of the work force. In the 1940s and 1950s, women continued their march into the workplace since traditional feminine roles of homemaker and mother (AP, 832). In the post war, young men and women tight up after they got back from the war. Baby boom is the huge explosion from births to returningShow MoreRelatedThe Civil Rights Movement During World War II955 Words   |  4 PagesDuring World War II, America as a nation faced many challenges both at home and abroad. Some of these challenges at home included Strikes and protests in war production factories, which was due to increasing differences between the government and employers, with the workers/labor unions. 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